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  Indian J Med Microbiol
 

Figure 5: MiR-375 and miR-5691 regulate hypertrophic scar formation through targeting thrombospondin 1. (a) The transfection efficiency of si-thrombospondin 1 in hypertrophic scar fibroblasthypertrophic scar fibroblasts was analyzed by reverse transcription-polymerase chain reaction. hypertrophic scar fibroblasts with the transfection of si-thrombospondin-1 alone or combined with miR-375/miR-5691 inhibitors for 48 h before subsequent experiments. (b) 3-(4,5)-dimethylthiahiazo (-z-y1)-2,5-di-phenytetrazoliumromide assay examined the proliferation of hypertrophic scar fibroblasts with diverse transfections. (c) The expression levels of caspase-3, caspase-9, C-caspase-3, C-caspase-9, Bax, and Bcl-2 in hypertrophic scar fibroblasts with diverse transfections were detected by western blotting. (d) The expression levels of proliferating cell nuclear antigen, transforming growth factor beta-1, alpha-smooth muscle actin, fibronectin, collagen type I, and collagen type III in hypertrophic scar fibroblasts with diverse transfections were measured by Western blotting. Note: *P < 0.05 and **P < 0.01.

Figure 5: MiR-375 and miR-5691 regulate hypertrophic scar formation through targeting thrombospondin 1. (a) The transfection efficiency of si-thrombospondin 1 in hypertrophic scar fibroblasthypertrophic scar fibroblasts was analyzed by reverse transcription-polymerase chain reaction. hypertrophic scar fibroblasts with the transfection of si-thrombospondin-1 alone or combined with miR-375/miR-5691 inhibitors for 48 h before subsequent experiments. (b) 3-(4,5)-dimethylthiahiazo (-z-y1)-2,5-di-phenytetrazoliumromide assay examined the proliferation of hypertrophic scar fibroblasts with diverse transfections. (c) The expression levels of caspase-3, caspase-9, C-caspase-3, C-caspase-9, Bax, and Bcl-2 in hypertrophic scar fibroblasts with diverse transfections were detected by western blotting. (d) The expression levels of proliferating cell nuclear antigen, transforming growth factor beta-1, alpha-smooth muscle actin, fibronectin, collagen type I, and collagen type III in hypertrophic scar fibroblasts with diverse transfections were measured by Western blotting. Note: *<i>P</i> < 0.05 and **<i>P</i> < 0.01.