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  Indian J Med Microbiol
 

Figure 3: Silencing of miR-375 or miR-5691 suppresses the hypertrophic scar phenotypes in hypertrophic scar fibroblasts. (a) Transfection efficiency of (left) miR-375 inhibitors and (right) miR-5691 inhibitors in hypertrophic scar fibroblasts was analyzed by reverse transcription-polymerase chain reaction. (b) 3-(4,5)-dimethylthiahiazo (-z-y1)-2,5-di-phenytetrazoliumromide assay examined the proliferation of hypertrophic scar fibroblasts transfected with (left) miR-375 inhibitors and (right) miR-5691 inhibitors. (c) The expression levels of caspase-3, caspase-9, C-caspase-3, C-caspase-9, Bax, and Bcl-2 in hypertrophic scar fibroblasts with diverse transfections were detected by western blot assay. (d) The expression levels of proliferating cell nuclear antigen, transforming growth factor beta-1, alpha-smooth muscle actin, fibronectin, collagen type I, and collagen type III in hypertrophic scar fibroblasts with diverse transfections were determined by Western blot assay. Note: *P < 0.05 and **P < 0.01.

Figure 3: Silencing of miR-375 or miR-5691 suppresses the hypertrophic scar phenotypes in hypertrophic scar fibroblasts. (a) Transfection efficiency of (left) miR-375 inhibitors and (right) miR-5691 inhibitors in hypertrophic scar fibroblasts was analyzed by reverse transcription-polymerase chain reaction. (b) 3-(4,5)-dimethylthiahiazo (-z-y1)-2,5-di-phenytetrazoliumromide assay examined the proliferation of hypertrophic scar fibroblasts transfected with (left) miR-375 inhibitors and (right) miR-5691 inhibitors. (c) The expression levels of caspase-3, caspase-9, C-caspase-3, C-caspase-9, Bax, and Bcl-2 in hypertrophic scar fibroblasts with diverse transfections were detected by western blot assay. (d) The expression levels of proliferating cell nuclear antigen, transforming growth factor beta-1, alpha-smooth muscle actin, fibronectin, collagen type I, and collagen type III in hypertrophic scar fibroblasts with diverse transfections were determined by Western blot assay. Note: *<i>P</i> < 0.05 and **<i>P</i> < 0.01.